A month after I started the construction of transcriptional fusions have obtained experimental evidence that they are ready. Had taken less, but over the intermediate plasmid and a restriction Ezim and had to wait to fill them. The left today all set to organize a bacterial orgy with which to move my mergers E. coli to R. etli and three days after the first experiment will serve qualitative know where we stand. I hope my promoter locate where 5 years of experimental results predict that will be, but like any experimental biologist knows: Life Happens ....
hope not "Happens to me" pa already run out of posts cryptic.
In the picture accompanying this post the result of PCR was used to verify Cloning correct my mergers. The first 6 lines are the experimental samples, the seventh and ninth are the controls which, along with the size of smaller streaks that are on my experimental samples confirms ue my fragments were cloned into the vector in the direction indicated. Also I digestion re-affirms that this outcome and costs and entered in sequence nomas pa be 100% sure;)
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